Catalog Number - 70000-15D

Laboratory‑Standard Bio‑Inert Diamond Hydride™ Performance

The 70000‑15D Bio‑Inert Diamond Hydride™ HPLC Column is a custom‑manufactured analytical column designed for laboratories requiring maximum protection from analyte–metal interactions without sacrificing performance. Built on Cogent TYPE‑C™ silica hydride technology and packed in metal‑free stainless steel hardware and frits, this column delivers stable retention, excellent selectivity, and long service life for challenging polar and biologically relevant compounds.

The classic 4.6 mm ID × 150 mm length represents the most widely adopted analytical HPLC format, offering an optimal balance of resolution, robustness, and method flexibility on standard HPLC instrumentation.

Why This Bio‑Inert Configuration Is Selected

This column format is widely chosen when analytical integrity and reproducibility are critical:

• High resolving power for complex polar mixtures

• Excellent peak shape and injection‑to‑injection repeatability

• Compatibility with conventional HPLC pumps and UV detectors

• Support for higher injection volumes without specialized system requirements

The bio‑inert, metal‑free internal surfaces reduce unwanted adsorption, degradation, or loss of metal‑sensitive analytes, improving accuracy and quantitative reliability.

Diamond Hydride™ Stationary Phase Advantage

Manufactured using Cogent TYPE‑C™ silica hydride technology, the Diamond Hydride™ stationary phase offers fundamental advantages over traditional bonded‑silica HILIC phases:

• Rapid equilibration, minimizing downtime between injections

• Highly stable and reproducible retention, even at high organic content

• Mixed‑mode interaction capability for difficult or poorly retained analytes

• Extended column lifetime suitable for routine and regulated environments

These properties make Diamond Hydride™ columns especially effective for methods requiring consistency across long analytical campaigns.

Ideal Applications

The 70000‑15D bio‑inert Diamond Hydride column is routinely used for:

• Small, highly polar molecules

• Amino acids and organic acids

• Polar and moderately non‑polar peptides

• Proteins, metabolites, and other biologically relevant compounds

It is particularly valuable for analytes known to interact with conventional stainless‑steel column hardware.

High‑Resolution HPLC and UV Detection

The 150 mm column length provides increased plate count for improved separation of closely eluting compounds and is well‑suited for:

• Fast analysis of highly polar compounds using UV detection

• Methods where shorter columns do not provide sufficient resolution

• Validated and regulated analyses requiring robustness and reproducibility

Because of its standard 4.6 mm internal diameter, the column integrates seamlessly with traditional detector configurations and autosamplers.

LC‑MS Compatibility

While optimized for conventional HPLC workflows, the 70000‑15D is fully compatible with LC‑MS applications where standard analytical flow rates are acceptable or where post‑column flow splitting is employed. The bio‑inert construction further supports MS workflows by minimizing metal‑related artifacts.

Operating Guidelines and System Requirements

• Typical flow‑rate range: ~0.8–1.5 mL/min (method‑ and solvent‑dependent)

• System compatibility: All standard analytical HPLC systems

• Special requirements: No low‑flow capability required

Flow rates may be adjusted based on pressure limits, mobile‑phase viscosity, and resolution objectives.

Custom Item Notice

This product is classified as a Custom Item. Custom columns are made to order, non‑returnable, and cannot be canceled once production begins.

• Learn more about Custom Orders and our Custom Order policies.